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Environmental Science Archives

e-ISSN: 2583-5092


ESA News

1 January 2024

A Study on Microbial Carriage on Door Handles

Odo JI et al., 2024

As people come in contact with surfaces like keyboards, office furniture, toilet doors, buses and keke door handles, there is a probability of picking up microbes deposited on them. This study is aimed at evaluating the antibiogram and microbial carriage of campus buses and keke napep door handles. Thirty swab samples were obtained from buses and keke napep door handles. The samples were taken to the laboratory where they were serially diluted and inoculated. Identification, characterization and biochemical analysis were done using standard microbiological methods. Fungal colonies were macroscopically and microscopically examined for morphology and appearance. Bacterial isolated were identified to be Staphylococcus spp., Proteus spp., Escherichia coli and Shigella spp. Fungi isolated were identified to be Aspergillus niger, Mucor spp., Aspergillus fumigates, Rhizopus spp. and Yeast spp. This study has shown that for both bacterial and fungal species, Staphylococcus spp.5(33.33%) and Aspergillus niger 2(50%) had the highest occurrence while Proteus spp. 1(6.67%), Aspergillus fumigates and Mucor spp. 1(14.29%) had the least occurrence. Results of susceptibility test carried out on bacteria isolates show Staphylococcus spp. was most susceptible to levofloxacin having 21.67 ± 2.89 mean zone of inhibition and was resistant to rifampicin which had no zone of inhibition. Furthermore, using Gram-negative disc, all the isolates were susceptible to the antibiotics used except Proteus spp. which showed resistance to ciporex and nalidixic acid. Data was further analyzed at P<0.05 using ANOVA. The analysis shows that there was no significant difference (P>0.05) in the susceptibility analysis. This study has shown that Campus Buses door handles have higher microbial carriage than keke door handles though both can serve as a means of microbial carriage and vehicle for the transmission of disease since they are associated with pathogenic organisms thus, hygienic measures should be taken to avoid the transmission of diseases by these public surfaces in which contact is constantly made with.

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