How helpful are these tests for acute toxicity, has been questioned by many researchers. Numerous ecotoxicologists claim that the advantages of these tests lie in their ability to recreate certain environmental conditions in the lab, which allows for the evaluation of acute toxicity afterward. Whether these acute tests provide useful information on how a certain substance affects individuals and communities is a related question. The other species are not taken into consideration if testing is done on a specific species. Because of this, there is always some debate over their accuracy or the usefulness of extrapolating from one species to another. But that study on a single species, provides a piece of information which will be helpful in creating the compilation of toxic effects of a particular compound on different species. 

The knowledge of oxidative stress in fish and other animals has a great importance in the environmental and aquatic toxicological studies. Oxidative stress is evoked by many chemicals, including some pesticides, metals and other organic pollutants. Antioxidant defense systems in fish and other animals can be used to assess a specific area of toxicity. The present understanding of the role played by numerous chemical and environmental toxins in the onset of oxidative stress in the gonads of fishes is summarized in this article. These toxins play a significant role in the development of reactive oxygen species by inducing oxidative stress in both aquatic and terrestrial organisms. Reactive oxygen species are produced in excess, which causes oxidative damage such as lipid peroxidation, protein and DNA oxidation, and enzyme inactivation. This review paper reveals how different natural and artificial toxins affect the reproductive capability and capacity of fishes, by showing how these toxins initiate oxidative stress and how in turn affect the structure, anatomy, physiology and functioning of gonads as well as reproductive cells. Antioxidant defense mechanisms are employed as biochemical indicators of oxidative stress. The study of these biomarkers and their effects can be used for biomonitoring the level of environmental toxicity in different organisms.

Over recent decades, environmental pollution is rapidly increasing because of the anthropogenic activities of uncontrolled development, for example, industry, transport, agriculture, and urbanization, which generates harmful contaminants for living organisms including humans. These contaminants get accumulated in the organism via different routes and get bioaccumulated in different tissues exerting detrimental effects at different levels (molecular, cellular and physiological levels). The measurement of biological assays in sentinel species to access quality and changes of the environment is known as environmental biomonitoring which provides an indication of environmental stress. Several biomarkers evaluate the nature and extent of the exposure and evaluation of adverse biological responses to pollutants in a biomonitoring program of the aquatic environment. These include behavioral response, genotoxicity (comet assay and micronucleus assay) and oxidative stress. Here we present the importance of these biomarkers.

Heavy metal toxicity is a profound reason for the decline in population of insect pollinators as it transfers through food chain and affect the physiological aspect of the insects. Thus, the heavy metal analysis in different insects such as butterfly (Pieris rapae), honey bee (Apis dorsata) and arachnid spider (Pholcus phalangioides) were examined by using atomic absorption spectrophotometer. The aim of the study was to analyse the contamination and accumulation of heavy metals (Hg, Pb, Cr, Cd, Ni) in insect species as ecological indicator. It was revealed that the mercury (Hg) was in the highest concentration in all the insect samples and the nickel in lowest concentration. Data was statistically evaluated using one way ANOVA and post hoc analysis with significant value 0.05 was applied in case of p<0.05 suggested that all insects were good ecological indicator. There was no significant difference in lead, chromium and nickel concentration of honey bees, spiders and butterflies. Honey bees varied significantly p< 0.02 with the spiders and butterflies for cadmium. For mercury, the spiders and honey bees were non-significant and the butterflies were significant p< 0.03 with the spiders and honey bees. Present study indicated that heavy metals contamination is the major environmental and health concern in Pakistan and insects can be used as bioindicator for environmental pollution. 

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