top of page
Windows 7 ultimate collection of wallpapers (60).jpg

Serological Detection of Viruses causing Tomato Mosaic Disease

Stanzin Diskit 1, LV Ravishankar 1, Ranbir Singh 1, Bushra Rasool 1, Dechan Choskit 1, Nisar Ahmad Dar 1, Amrish Vaid 1, Sachin Gupta 1 and Baby Summuna 2

2024/06/02

DOI: 10.5281/zenodo.11424734

ABSTRACT

Tomato (Solanum lycopersicum L.) belongs to solanaceous family and is sensitive to many plant viruses during the different crop stages and viruses are spread by insect vectors. Mostly plant viruses with ss-RNA infect the crop vulnerability and cause huge losses, including Tomato mosaic virus (ToMV), Cucumber mosaic virus (CMV), and Potato mosaic virus (PVY). The survey was conducted in the subtropical climate zone of Jammu province in different locations viz. Gajansoo, Pinjore, and Lalyal from Marh, Badayal Brahmna, Laswara and Purobana from RS Pura, Makhanpur, Salehar, and Kothe from Bishnah and Chatha Farm during 2020 and 2021. It was observed that the overall incidence of disease in Marh was 32.44% in Gajansoo and a minimum (16.67%) in Makhanpur of Bishnah during 2020 while during 2021, a maximum disease incidence of 30.66% was recorded from Gajansoo of Marh and minimum (15.75%) was recorded from Purobana of RS Pura. Host resistance for different plant viruses has always been a successful management strategy in the control of plant viral diseases, which are ecologically and economically valid. Twenty germplasms of tomato viz. Pusa Ruby, EC-620406, Arka Vikas, EC-771607, EC-676791, Hisar Anmol, EC- 514109, EC- 514109, EC-677191, EC-677049, EC- 677123, Avinash 2, Arka Sourabh, Kashi Vishes, Local, Kajal, Hiasr Arun, Money Maker, EC-620417, Arka Ananya, EC-617048 obtained from NBPGR were screened against ToMV, CMV and PVY under field conditions to know the host resistance to three plant viruses which are detected serologically through DAS-ELISA.

AUTHOR AFFILIATIONS

1 Division of Plant Pathology, FoA, SKUAST - Jammu - 180009, Jammu and Kashmir, India
2 Division of Plant Pathology, FoH, SKUAST - Kashmir -190025, Jammu and Kashmir, India

29111_edited.jpg

CITATION

Diskit S, Ravishankar LV, Singh R, Rasool B, Choskit D, Dar NA, Vaid A, Gupta S and Summuna B (2024) Serological Detection of Viruses causing Tomato Mosaic Disease. Environ Sci Arch 3(STI-1): 27-36.

REFERENCES

Ali A and Kobayashi M (2010) Seed transmission of Cucumber mosaic virus in pepper. Journal of Virological Methods 163: 234-237.
Almeida JEM, Figueira ADR, Duarte PDSG, Lucas MA and Alencar NE (2018) Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis. Bragantia 77: 590-598.
Balogun OS, Xu L, Teraoka T and Hosokawa D (2002) Effects of single and double infections with Potato virus X and Tobacco mosaic virus on disease development, plant growth, and virus accumulation in tomato. Fitopatologia Brasileira 27: 241-248.
Blancard D (2012) Principal characteristics of pathogenic agents and methods of control. Tomato Diseases 413-650.
Faurez F, Baldwin T, Tribodet M and Jacquot E (2012) Identification of new Potato virus Y (PVY) molecular determinants for the induction of vein necrosis in tobacco. Molecular plant pathology 13: 948-959.
Ghosh A, Rao G and Baranwal V (2019) Manual on transmission of plant viruses and phytoplasmas by insect vectors. Indian Agricultural Research Institute, New Delhi 110012, 60.
Hančinský R, Mihálik D, Mrkvová M, Candresse T and Glasa M (2020) Plant viruses infecting Solanaceae family members in the cultivated and wild environments: a review. Plants 9: 667.
Herrera-Vasquez J, Alfaro-Fernández A, Cordoba-Selles M, Cebrian M, Font M and Jordá C (2009) First report of Tomato torrado virus infecting tomato in single and mixed infections with Cucumber mosaic virus in Panama. Plant Disease 93: 198-198.
Kang LY, Yang XC and Tien P (1982). Double-stranded viral RNA content in tobacco leaves infected with virulent and avirulent isolates of tomato mosaic virus. Virology 118: 324-328.
Mrkvová M, Hančinský R, Grešíková S, Kaňuková Š, Barilla J, Glasa M, Hauptvogel P, Kraic J and Mihálik D (2022) Evaluation of new polyclonal antibody developed for serological diagnostics of tomato mosaic virus. Viruses 14: 1331.
Nadeem S, Ullah N, Akhtar KP, Hameed A and Saleem MY (2022) Evaluation of Tomato Hybrids for Resistance against Tomato Mosaic Virus (ToMV). Journal of Botanical Research 4: 1-11.
Nazir S, Javed MA, Jamil MW, Habib I and Iqbal MZ 2018 Synergistic Effects of Mosaic and Leaf Curl Viruses on Growth in Glass-House Tomato Plants.
Nikolić D, Vučurović A, Stanković I, Radović N, Zečević K, Bulajić A and Krstić B (2018) Viruses affecting tomato crops in Serbia. European Journal of Plant Pathology 152: 225-235.
Panno S, Davino S, Caruso AG, Bertacca S, Crnogorac A, Mandić A, Noris E and Matić S (2021) A review of the most common and economically important diseases that undermine the cultivation of tomato crop in the mediterranean basin. Agronomy 11: 2188.
Park KH and Cha BJ (2002) Detection of TMV, ToMV, and CMV from tomato seeds and plants. Research in Plant Disease 8: 101-106.
Sarwar M (2020) Insects as transport devices of plant viruses. In "Applied Plant Virology", pp. 381-402. Elsevier.
Shehata WF, Iqbal Z, Abdelbaset TE, Saker KI, El Shorbagy AE, Soliman AM, Sattar MN and El-Ganainy SM (2023) Identification of a Cucumber Mosaic Virus from Cucurbita pepo on New Reclamation Land in Egypt and the Changes Induced in Pumpkin Plants. Sustainability 15: 9751.
Soler S, Prohens J, López C, Aramburu J, Galipienso L and Nuez F (2010) Viruses infecting tomato in Valencia, Spain: occurrence, distribution and effect of seed origin. Journal of Phytopathology 158: 797-805.
Song XS, Wang YJ, Mao WH, Shi K, Zhou YH, Nogués S and Yu JQ (2009) Effects of cucumber mosaic virus infection on electron transport and antioxidant system in chloroplasts and mitochondria of cucumber and tomato leaves. Physiologia Plantarum 135: 246-257.
Sulistyowati E, Mitter N, Bastiaan-Net S, Roossinck MJ and Dietzgen RG (2004) Host range, symptom expression and RNA 3 sequence analyses of six Australian strains of Cucumber mosaic virus. Australasian Plant Pathology 33: 505-512.
Tolin SA and Fayad A (2016) Virus diseases of tropical vegetable crops and their management. Integrated Pest Management of Tropical Vegetable Crops 41-76.
Verchot J (2022) Potato virus X: A global potato‐infecting virus and type member of the Potexvirus genus. Molecular Plant Pathology 23: 315-320.

by.png

License: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third-party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Visit for more details http://creativecommons.org/licenses/by/4.0/.

Reporter Name

Date

Date

bottom of page