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Nanopesticides and ‘All-Organic’ Nanopesticide Synthesis, Classification and their Use in Agriculture

Mehra S Sidhu and Subash Singh

2025/05/19

DOI: 10.5281/zenodo.15464794

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ABSTRACT

Two of the most significant difficulties that the new millennium presents are the achievement of sustainable agricultural productivity and the improvement of global food security. When it comes to addressing these difficulties, innovative technologies are required. These technologies should be able to boost global food production while simultaneously limiting collateral environmental harm and conserving the resilience of agroecosystems against a climate that is changing rapidly. When compared to conventional pesticides, nanomaterials that can encapsulate and deliver pesticidal active ingredients (AIs) responsively (for instance, regulated, targeted, and synchronized) present new options to boost the efficacy and efficiency of traditional pesticides. Although there are benefits to using nanoparticles in standard nanopesticide formulations, current studies indicate that some of these nanoparticles can harm crops and beneficial creatures. This toxicity is caused by the accumulation of nanoparticles in the organisms and their passage via the food chain. Consequently, conventional nanopesticides are considered disadvantageous for "green agriculture". When evaluating the present circumstances, creating "all-organic" nanopesticides might serve as an advanced solution for mitigating the harmful effects of conventional nanopesticides. Nevertheless, their understanding and practical use of concepts are significantly restricted. All-organic nanoparticles synthesized using green methods are more ecologically benign than traditional nanopesticides since they have low residue and dangerous effects. This research examines the present state of development of 'all-organic' nanomaterials and their potential as organic nanopesticides on target species compared to conventional nanopesticides.

AUTHOR AFFILIATIONS

1 Electron Microscopy and Nanoscience Lab, Directorate of Research, Punjab Agricultural University, Ludhiana, Punjab, India 141 004
2 School of Organic Farming, Punjab Agricultural University, Ludhiana, Punjab, India 141 004

CITATION

Sidhu MS and Singh S (2025) Nanopesticides and ‘All-Organic’ Nanopesticide Synthesis, Classification and their Use in Agriculture. Environmental Science Archives 4(1): 274-288.

REFERENCES

Abu Hazafa A, Murad M, Masood MU, et al. (2021) Nano-Biopesticides as an Emerging Technology for Pest Management . IntechOpen. https://doi.org/10.5772/intechopen.101285

Ahmad I, Irfan S, Dera AA, et al. (2020) GC-MS analysis of ethanol extract from aerial parts of Nepeta deflersiana and its anticancer and antimicrobial efficacies. Biologia (Bratislava) 75: 1739–1750. https://doi.org/10.2478/s11756-020-00473-3

Anjali CH, Sudheer Khan S, Margulis-Goshen K, et al. (2010) Formulation of water-dispersible nanopermethrin for larvicidal applications. Ecotoxicology and Environmental Safety 73: 1932–1936. https://doi.org/10.1016/j.ecoenv.2010.08.039

Awad M, Ibrahim E-DS, Osman EI, et al. (2022) Nano-insecticides against the black cutworm Agrotis ipsilon (Lepidoptera: Noctuidae): Toxicity, development, enzyme activity, and DNA mutagenicity. PLOS ONE 17: e0254285. https://doi.org/10.1371/journal.pone.0254285

Ayoub HA, Khairy M, Rashwan FA, Abdel-Hafez HF (2017) Synthesis and characterization of silica nanostructures for cotton leaf worm control. Journal of Nanostructure Chemistry 7: 91–100. https://doi.org/10.1007/s40097-017-0229-2

Bourguet D and Guillemaud T (2016) The hidden and external costs of pesticide use. In Lichtfouse E (ed.) Sustainable Agriculture Reviews: Volume 19 , pp. 35–120. Cham: Springer International Publishing.

Camara MC, Campos EVR, Monteiro RA, et al. (2019) Development of stimuli responsive nano-based pesticides: Emerging opportunities for agriculture. Journal of Nanobiotechnology 17: 100. https://doi.org/10.1186/s12951-019-0533-8

Chandel S (2021) Bio-Rational Pesticides: Alternative Pest Control Strategies. Just Agriculture 1(6): 1–5. https://www.justagriculture.in

Chen H and Yada RY (2011) Nanotechnologies in agriculture: New tools for sustainable development. Trends in Food Science & Technology 22(11): 585–594.

Chen K, Fu Z, Wang M, et al. (2018) Preparation and characterization of size-controlled nanoparticles for high-loading λ-cyhalothrin delivery through flash nanoprecipitation. Journal of Agricultural and Food Chemistry 66: 8246–8252. https://doi.org/10.1021/acs.jafc.8b02851

Côa F, Bortolozzo LS, Petry R, et al. (2020) Environmental toxicity of nanopesticides against non-target organisms: The state of the art. In Fraceto LF, de Castro SS, Grillo R, Ávila D, Oliveira HC, et al. (eds.) Nanopesticides: From Research and Development to Mechanisms of Action and Sustainable Use in Agriculture , pp. 227–279. Cham: Springer International Publishing.

Daniel MC and Astruc D (2004) Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chemical Reviews 104: 293–346.

Debnath N, Das S, Seth D, et al. (2011) Entomotoxic effect of silica nanoparticles against Sitophilus oryzae (L.). Journal of Pest Science 84: 99–105. https://doi.org/10.1007/s10340-010-0332-3

El-Naggar ME, Abdelsalam NR, Fouda MMG, et al. (2020) Soil application of nano silica on maize yield and its insecticidal activity against some stored insects after the post-harvest. Nanomaterials 10: 739. https://doi.org/10.3390/nano10040739

Elmer WH, et al. (2021) Foliar application of copper oxide nanoparticles suppresses Fusarium wilt development on chrysanthemum. Environmental Science & Technology 55: 10805–10810.

European Commission Joint Research Center (2010) Reference Report: Considerations on a Definition of Nanomaterial for Regulatory Purposes . Available at: http://ec.europa.eu/dgs/jrc/downloads/jrc_reference_report_201007_nanomaterials.pdf

FAO (2019) New Standards to Curb the Global Spread of Plant Pests and Diseases . Available at: https://www.fao.org/news/story/en/item/1187738/icode

FAO, IFAD, UNICEF, WFP, WHO (2019) The State of Food Security and Nutrition in the World 2019: Safeguarding Against Economic Slowdowns and Downturns . Available online: https://www.wfp.org/publications/2019-state-food-security-and-nutrition-world-sofi-safeguarding-against-economic

Farmery AK, Allison EH, Andrew NL, et al. (2021) Blind spots in visions of a “Blue Economy” could undermine the Ocean’s contribution to eliminating hunger and malnutrition. One Earth 4: 28–38.

Frederiksen HK, Kristensen HG, Pedersen M (2003) Solid lipid microparticle formulations of the pyrethroid gamma-cyhalothrin – incompatibility of the lipid and the pyrethroid and biological properties of the formulations. Journal of Controlled Release 86: 243–252. https://doi.org/10.1016/S0168-3659 (02)00406-6

Gao Y, Zhang Y, He S, et al. (2019) Fabrication of a hollow mesoporous silica hybrid to improve the targeting of a pesticide. Chemical Engineering Journal 364: 361–369. https://doi.org/10.1016/j.cej.2019.01.105

Gomez A, et al. (2020) Effects of nano-enabled agricultural strategies on food quality: current knowledge and future research needs. Journal of Hazardous Materials 401: 123385.

Green JM and Beestman GB (2007) Recently patented and commercialized formulation and adjuvant technology. Crop Protection 26: 320–327.

Gutierrez JM, Gonzalez C, Maestro A, et al. (2008) Nano-emulsions: New applications and optimization of their preparation. Current Opinion in Colloid & Interface Science 13: 245–251.

Horn D and Rieger J (2001) Organic nanoparticles in the aqueous phase: Theory, experiment, and use. Angewandte Chemie International Edition 40: 4331–4361.

Horowitz AR, Ellsworth PC, Ishaaya I (2009) Biorational Pest Control – An Overview . Springer.

Ishaque M, Schnabel G, Anspaugh DD (2013) Agrochemical formulations comprising a pesticide, an organic UV-photoprotective filter and coated metal-oxide nanoparticles. US Patent 8,404,263 B2.

Jameel M, Shoeb M, Khan MT, et al. (2020) Enhanced insecticidal activity of thiamethoxam by zinc oxide nanoparticles: A novel nanotechnology approach for pest control. ACS Omega 5: 1607–1615. https://doi.org/10.1021/acsomega.9b03680

Kah M (2015) Nanopesticides and nanofertilizers: Emerging contaminants. In Melo RA, Ariza P, Lissbrant S, et al. (eds.) Evaluation of agrochemicals and bioinputs for sustainable bean management on the Caribbean coast of Colombia . Agronomía Colombiana 33: 203–211.

Kah M, Beulke S, Tiede K, Hofmann T (2013) Nanopesticides: State of knowledge, environmental fate, and exposure modeling. Critical Reviews in Environmental Science and Technology 43: 1823–1867.

Kang H, et al. (2021) Silica nanoparticle dissolution rate controls the suppression of Fusarium wilt of watermelon (Citrullus lanatus ). Environmental Science & Technology 55: 13513–13522.

Kariyanna B, Prabhuraj A, Asokan R, et al. (2020) Genome mining and expression analysis of carboxylesterase and glutathione S-transferase genes involved in insecticide resistance in eggplant shoot and fruit borer, Leucinodes orbonalis (Lepidoptera: Crambidae). Frontiers in Physiology 11: 594845. https://doi.org/10.3389/fphys.2020.594845

Kaziem AE, Gao Y, Zhang Y, et al. (2018) α-Amylase triggered carriers based on cyclodextrin anchored hollow mesoporous silica for enhancing insecticidal activity of avermectin against Plutella xylostella . Journal of Hazardous Materials 359: 213–221. https://doi.org/10.1016/j.jhazmat.2018.07.059

Knowles A (2005) New developments in crop protection product formulation. Agrow Report . Available at: http://www.agrow.com/multimedia/archive/00068/DS24368749a.pdf

Kookana RS, Boxall ABA, Reeves PT, et al. (2014) Nanopesticides: Guiding principles for regulatory evaluation of environmental risks. Journal of Agricultural and Food Chemistry 62: 4227–4240. https://doi.org/10.1021/jf500232f

Kumar S, Chauhan N, Gopal M, et al. (2015) Development and evaluation of alginate–chitosan nanocapsules for controlled release of acetamiprid. International Journal of Biological Macromolecules 81: 631–637. https://doi.org/10.1016/j.ijbiomac.2015.08.062

Kumar S, Nehra M, Dilbaghi N, et al. (2019) Nano-based smart pesticide formulations: Emerging opportunities for agriculture. Journal of Controlled Release 294: 131–153. https://doi.org/10.1016/j.jconrel.2018.12.012

Lengai GMW, Muthomi JW, Mbega ER (2020) Phytochemical activity and role of botanical pesticides in pest management for sustainable agricultural crop production. Scientific African 7: e00239. https://doi.org/10.1016/j.sciaf.2019.e00239

Liu Y, Tong Z, Prud’homme RK (2008) Stabilized polymeric nanoparticles for controlled and efficient release of bifenthrin. Pest Management Science 64: 808–812. https://doi.org/10.1002/ps.1566

Lowry GV, Avellan A, Gilbertson LM (2019) Opportunities and challenges for nanotechnology in the agri-tech revolution. Nature Nanotechnology 14: 517–522.

Ma C, et al. (2021) Role of nanoscale hydroxyapatite in disease suppression of Fusarium -infected tomato. Environmental Science & Technology 55: 13465–13476.

Manna S, Roy S, Dolai A, Ravula AR, Perumal V, Das A (2023) Current and future prospects of “all-organic” nanoinsecticides for agricultural insect pest management. Frontiers in Nanotechnologies 4: 1082128. https://doi.org/10.3389/fnano.2022.1082128

McKee MS and Filser J (2016) Impacts of metal-based engineered nanomaterials on soil communities. Environmental Science: Nano 3: 506–533.

Mustafa IF and Hussein MZ (2020) Synthesis and technology of nanoemulsion-based pesticide formulation. Nanomaterials 10: 1608. https://doi.org/10.3390/nano10081608

Nuruzzaman Md, Rahman MM, Liu Y, Naidu R (2016) Nanoencapsulation, nano-guard for pesticides: a new window for safe application. Journal of Agricultural and Food Chemistry 64(7): 1447–1483.

OECD (2002) Guidelines for the Testing of Chemicals Test No. 307: Aerobic and Anaerobic Transformation in Soils. Paris: OECD Publishing.

Pan X, Guo X, Zhai T, et al. (2023) Nanobiopesticides in Sustainable Agriculture: Developments, Challenges, and Perspectives. Environmental Science: Nano.

Paramo LA, Feregrino-Pérez AA, Guevara R, et al. (2020) Nanoparticles in agroindustry: Applications, toxicity, challenges, and trends. Nanomaterials 10: 1654. https://doi.org/10.3390/nano10091654

Patel S, Bajpai J, Saini R, et al. (2018) Sustained release of pesticide (Cypermethrin) from nanocarriers: An effective technique for environmental and crop protection. Process Safety and Environmental Protection 117: 315–325. https://doi.org/10.1016/j.psep.2018.05.012

Pavela R (2014) Limitation of plant biopesticides. In Advances in Plant Biopesticides , pp. 347–359. Springer. https://doi.org/10.1007/978-81-322-2006-0_17

Rajeshwari A, Suresh S, Chandrasekaran N, Mukherjee A (2016) Toxicity evaluation of gold nanoparticles using an Allium cepa bioassay. RSC Advances 6: 24000–24009. https://doi.org/10.1039/C6RA04712B

Rawat S, et al. (2021) Soil-weathered CuO nanoparticles compromise foliar health and pigment production in spinach (Spinacia oleracea ). Environmental Science & Technology 55: 13504–13512.

Sabry AH, Salem HA-N, Metwally HM (2021) Development of imidacloprid and indoxacarb formulations to nanoformulations and their efficacy against Spodoptera littoralis (Boisd). Bulletin of the National Research Centre 45: 16. https://doi.org/10.1186/s42269-020-00477-8

Saini P, Gopal M, Kumar R, Srivastava C (2014) Development of pyridalyl nanocapsule suspension for efficient management of tomato fruit and shoot borer (Helicoverpa armigera ). Journal of Environmental Science and Health Part B 49: 344–351. https://doi.org/10.1080/03601234.2014.882168

Satehi AB, Ziaee M, Ashrafi A (2018) Silica nanoparticles: A potential carrier of chlorpyrifos in slurries to control two insect pests of stored products. Entomologia Generalis 37: 77–82.

Schäfer RB, Liess M, Altenburger R, et al. (2019) Future pesticide risk assessment: Narrowing the gap between intention and reality. Environmental Sciences Europe 31: 21. https://doi.org/10.1186/s12302-019-0203-3

Shang H, et al. (2021) Copper oxide nanoparticle-embedded hydrogels enhance nutrient supply and growth of lettuce (Lactuca sativa ) infected with Fusarium oxysporum f. sp. lactucae . Environmental Science & Technology 55: 13432–13442.

Sharma S, Sahu BK, Cao L, et al. (2021a) Porous nanomaterials: Main vein of agricultural nanotechnology. Progress in Materials Science 121: 100812. https://doi.org/10.1016/j.pmatsci.2021.100812

Sillen WMA, et al. (2020) Nanoparticle treatment of maize analyzed through the metatranscriptome: Compromised nitrogen cycling, possible phytopathogen selection, and plant hormesis. Microbiome 8: 127.

Singh A and Leppanen C (2020) Known target and nontarget effects of the novel neonicotinoid cycloxaprid to arthropods: A systematic review. Integrated Environmental Assessment and Management 16: 831–840. https://doi.org/10.1002/ieam.4305

Singh S and Sidhu MS (2025) Plant derivative extracts (‘Brahmastra’) spectral characterization and their impact on Lipaphis erysimi and natural enemies in oilseed-mustard under organic farming conditions. Organic Agriculture. https://doi.org/10.1007/s13165-025-00502-y

Sparks ME, Bansal R, Benoit JB, et al. (2020) Brown marmorated stink bug, Halyomorpha halys (Stål), genome: Putative underpinnings of polyphagy, insecticide resistance potential and biology of a top worldwide pest. BMC Genomics 21: 227. https://doi.org/10.1186/s12864-020-6510-7

Sparks TC (2013) Insecticide discovery: An evaluation and analysis. Pesticide Biochemistry and Physiology 107: 8–17. https://doi.org/10.1016/j.pestbp.2013.05.012

Srishailam B, Sailaja V, Nikhitha A, Kiran PK (2022) Promoting startups in Agriculture: An Innovative Approach for Transforming Agriculture to Agri-Business.

Stankovic S, Kostic M, Kostic I, Krnjajic S (2020) Practical approaches to pest control. In Kontogiannatos D, Kourti A, Mendes KF (eds.) Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production . London: IntechOpen.

Stanley S (2014) Biological nanoparticles and their influence on organisms. Current Opinion in Biotechnology 28: 69–74. https://doi.org/10.1016/j.copbio.2013.11.014

Sun L, Wang Y, Wang R, et al. (2020) Physiological, transcriptomic and metabolomic analyses reveal zinc oxide nanoparticles modulate plant growth in tomato. Environmental Science: Nano 7: 3587–3604.

Teng H, Yuan Y, Zhang T, et al. (2020) Evaluation of the sublethal effect of tetrachlorantraniliprole on Spodoptera exigua and its potential toxicity to two non-target organisms. PLOS ONE 15: e0242052. https://doi.org/10.1371/journal.pone.0242052

Vašíčková J, Hvězdová M, Kosubová P, Hofman J (2019) Ecological risk assessment of pesticide residues in arable soils of the Czech Republic. Chemosphere 216: 479–487. https://doi.org/10.1016/j.chemosphere.2018.10.158

Vinci G, Savastano M, Restuccia D, Ruggeri M (2025) Nanobiopesticides: Sustainability aspects and safety concerns. Environments 12(3): 74.

Wang C, Cui B, Zhao X, et al. (2019) Optimization and characterization of lambda-cyhalothrin solid nanodispersion by self-dispersing method. Pest Management Science 75: 380–389. https://doi.org/10.1002/ps.5122

Wang Y, Cui H, Sun C, Zhao X, Cui B (2014) Construction and evaluation of controlled-release delivery system of Abamectin using porous silica nanoparticles as carriers. Nanoscale Research Letters 9: 655. https://doi.org/10.1186/1556-276X-9-655

Wang Z, Yue L, Dhankher OP, Xing B (2020) Nano-enabled improvements of growth and nutritional quality in food plants driven by rhizosphere processes. Environment International 142: 105831.

Wheeler T and von Braun J (2013) Climate change impacts on global food security. Science 341: 508–513.

Xiang Y, Zhang G, Chen C, et al. (2018) Fabrication of a pH-responsively controlled-release pesticide using an attapulgite-based hydrogel. ACS Sustainable Chemistry & Engineering 6: 1192–1201. https://doi.org/10.1021/acssuschemeng.7b03469

Xu Z, Gao Z, Shao X (2018) Light-triggered release of insecticidally active spirotetramat-enol. Chinese Chemical Letters 29: 1648–1650. https://doi.org/10.1016/j.cclet.2018.01.025

Zhang H, Wang D, Butler R, et al. (2008) Formation and enhanced biocidal activity of water-dispersable organic nanoparticles. Nature Nanotechnology 3: 506–511. https://doi.org/10.1038/nnano.2008.188

Zhang W (2018) Global pesticide use: Profile, trend, cost/benefit and more. Proceedings of the International Academy of Ecology and Environmental Sciences 8: 1–27.

Zhang Y, Chen W, Jing M, et al. (2019) Self assembled mixed micelle loaded with natural pyrethrins as an intelligent nanoinsecticide with a novel temperature-responsive release mode. Chemical Engineering Journal 361: 1381–1391. https://doi.org/10.1016/j.cej.2018.10.132

Zhao L, Huang Y, Adeleye AS, Keller AA (2017) Metabolomics reveals Cu(OH)₂ nanopesticide-activated anti-oxidative pathways and decreased beneficial antioxidants in spinach leaves. Environmental Science & Technology 51: 10184–10194. https://doi.org/10.1021/acs.est.7b02163

Zhao L, Ortiz C, Adeleye AS, et al. (2016) Metabolomics to detect response of lettuce (Lactuca sativa ) to Cu(OH)₂ nanopesticides: Oxidative stress response and detoxification mechanisms. Environmental Science & Technology 50: 9697–9707. https://doi.org/10.1021/acs.est.6b02763

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