ABSTRACT
Many pathogenic microorganisms contribute to major human illnesses, such as infectious diseases, which are one of the most important concerns for humans. Due to treatment with antibiotics having drug resistance problems, in recent years, the use of new herbal and alternative medicines with fewer side effects is being considered for these types of infections. The objective of this study was to investigate the antibacterial activity of Rose (Rosa damascena), Marigold (Calendula officinalis), Tuberose (Polianthes tuberosa), Orchid (Dendrobium orchid), Blue pea (Clitoria ternatea) and Chrysanthemum (Chrysanthemum indica) extracts against test organisms- Staphylococcus aureus, Bacillus sp., Pseudomonas sp., Klebsiella sp., Vibrio sp. Six flower samples were collected in pre-sterilized zip-lock bags and transported to the Centre of Excellence Laboratory, Primeasia University at the earliest convenience. Antibacterial effects of aqueous, ethanol and acetone extracts of six flower samples were experimented on above mentioned reference strains post-extraction. Antimicrobial effects were investigated using well diffusion, as well as MIC and MBC. Phytochemical analyses of flower petals were conducted. The results showed that the ethanol extract of flowers inhibited the growth of organisms in comparison to ethanol, acetone and aqueous extract. Flower petals also showed the presence of various phytochemicals. This study justified that the flower petals, which are used only for ornamental purposes, also have antibacterial activity and used for the treatment of various diseases.
AUTHOR AFFILIATIONS
1 Department of Microbiology, Primeasia University, Dhaka 1214, Bangladesh
2 Ludwig-Maximilians-Universität München, Germany
3 Department of Microbiology, Bangladesh Jute Research Institute, Dhaka 1207, Bangladesh
CITATION
Abony M, Banik A, Bhowmik DR, Islam MR, Fariha NN and Ahmed Z (2025) Exploring the Antibacterial Potential of Traditional Medicinal Flowers- A Phytochemical and Microbiological Approach. Environmental Science Archives 4(2): 504-513.
REFERENCES
Amini MH, Ashraf K, Lim SM, et al. (2022) Phytochemical profiling, salt impurities removal and in vitro antibacterial evaluation of Calotropis procera twig, leaf and flower extracts. South African Journal of Botany 151. Elsevier: 367–378.
Avijit B, Zerin T and Rajia S (2020) Comparative phytochemical and antibacterial properties of Piper betle leave extracts from Barguna and Moheshkhali, Bangladesh. Iranian Journal of Medical Microbiology 14(2). Iranian Journal of Medical Microbiology: 125–132.
Banik A, Abony M, Zerin T, et al. (2018) Antibacterial activity of Allium sativum, Syzygium aromaticum, and Cinnamomum zeylanicum against food borne pathogens in vitro. IOSR Journal of Pharmacy and Biological Sciences 13: 68–73.
Bourgaud F, Gravot A, Milesi S, et al. (2001) Production of plant secondary metabolites: a historical perspective. Plant science 161(5). Elsevier: 839–851.
Buddhika HDK, Menuka Arawwawala LDA, Dharmadasa RM, et al. (2021) Phytochemical Properties of Clitoria ternatea L.(Fabaceae)-A Distinct Flower Morphometric Plants Available in Sri Lanka. In: 2021. 1st International Electronic Conference Agronomy Volume: 1.
Cahyaningrum PL and AAA SSW (2023) Antibacterial activity of Marigold flower (Tagetes Erecta L.) ethanol extract cream against staphylococcus aureus. J Voc Health Stud 6: 165–172.
Chandurkar P, Murab T, Ahakey N, et al. (2015) Antimicrobial activity of aqueous, acetone and methanol extracts of Calendula officinalis L.(Marigold) flower. International Journal of Pure & Applied Bioscience 3(2): 386–388.
Chowdhury SP, Banik A, Aurin SA, et al. (2019) Comparison of individual and synergistic antimicrobial activity of common spices against certain infectious pathogen in Bangladesh. American Journal of Plant Sciences 10(9). Scientific Research Publishing: 1599–1611.
Danton O, Somboro A, Fofana B, et al. (2019) Ethnopharmacological survey of plants used in the traditional treatment of pain conditions in Mali. Journal of Herbal Medicine 17. Elsevier: 100271.
FLIOU J, SPINOLA F, RIFFI O, et al. (2023) Phytochemical evaluation and in-vitro antibacterial activity of ethanolic extracts of Moroccan Lavandula x intermedia leaves and flowers. Biodiversitas Journal of Biological Diversity 24(10).
Górniak I, Bartoszewski R and Króliczewski J (2019) Comprehensive review of antimicrobial activities of plant flavonoids. Phytochemistry reviews 18. Springer: 241–272.
Horablaga NM, Cozma A, Alexa E, et al. (2023) Influence of sample preparation/extraction method on the phytochemical profile and antimicrobial activities of 12 commonly consumed medicinal plants in Romania. Applied Sciences 13(4). MDPI: 2530.
Jamil N and Pa’ee F (2018) Antimicrobial activity from leaf, flower, stem, and root of Clitoria ternatea–A review. In: AIP Conference proceedings, 2018. AIP Publishing.
Kumar M and More D (2019) Phytochemical analysis and bioactivity of selected medicinal plant of butterfly-pea (Clitoria ternatea L.) used by Kolam tribe Addjoing region of Telangana and Maharashtra states. Pharma Innov 8(1): 417–421.
Moliner C, Barros L, Dias MI, et al. (2018) Edible flowers of Tagetes erecta L. as functional ingredients: phenolic composition, antioxidant and protective effects on Caenorhabditis elegans. Nutrients 10(12). MDPI: 2002.
Munir N, Khilji SA, Rasool S, et al. (2025) Phytochemical Analysis, Antibacterial, and Antitumor Potential of Hibiscus rosa-sinensis Linn. Scientifica 2025(1). Wiley Online Library: 2722306.
Mutiara B, Christian YE and Setiawansyah A (2025) Post-harvest process influences antibacterial activity of Clitoria ternatea flower extracts against Staphylococcus aureus and Escherichia coli. Sasambo Journal of Pharmacy 6(1): 40–45.
Panche AN, Diwan AD and Chandra SR (2016) Flavonoids: an overview. Journal of nutritional science 5. Cambridge University Press: e47.
Patil RS, Harale PM, Shivangekar KV, et al. (2015) Phytochemical potential and in vitro antimicrobial activity of Piper betle Linn. leaf extracts. Journal of chemical and pharmaceutical Research 7(5): 1095–1101.
Safdar Y and Malik T (2020) Antibacterial activity of rose extract. Open Access journal of complementary and alternative medicine 2(4): 194–201.
Satria D, Sofyanti E, Wulandari P, et al. (2022) Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC® 25175TM and Staphylococcus aureus ATCC® 6538TM. Pharmacia 69. Bulgarian Pharmaceutical Scientific Society: 195–202.
Sharifi-Rad J, Sureda A, Tenore GC, et al. (2017) Biological activities of essential oils: From plant chemoecology to traditional healing systems. Molecules 22(1). MDPI: 70.
Sharma A, del Carmen Flores-Vallejo R, Cardoso-Taketa A, et al. (2017) Antibacterial activities of medicinal plants used in Mexican traditional medicine. Journal of ethnopharmacology 208. Elsevier: 264–329.
Tunç K, Semerci AB, İnceçayır D, et al. (2019) Antimicrobial activity of different flower extracts. Current Perspectives on Medicinal and Aromatic Plants 2(1). Nazım ŞEKEROĞLU: 53–58.
Zhou H, Chen L, Ouyang K, et al. (2023) Antibacterial activity and mechanism of flavonoids from Chimonanthus salicifolius SY Hu. and its transcriptome analysis against Staphylococcus aureus. Frontiers in Microbiology 13. Frontiers Media SA: 1103476.
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