top of page
Windows 7 ultimate collection of wallpapers (60).jpg

Study on Potentially Toxic Benthic Dinoflagellate Assemblages on Dead Corals in Nha Trang Bay, Vietnam

< Back

The V Ho, Nie B H’Yon, Phu H Le, Thu M Phan and Hoang K Phan

2025/09/30

DOI: 10.5281/zenodo.17235332

29111_edited.jpg

ABSTRACT

Dead branching coral samples were collected in Nha Trang Bay to isolate benthic dinoflagellates. The identification and taxonomic observation of benthic dinoflagellates were based on cell size, shape, surface morphology, and thecal plate structure using light microscopy and scanning electron microscopy. A total of 27 benthic dinoflagellate species belonging to 5 orders were identified from dead corals. The cell density of dinoflagellates varied spatially. During the dry season, the highest density reached 17.8 cells/cm², while in the rainy season, the highest density was 15.7 cells/cm². Five species of Gambierdiscus australes, G. carpenteri, G. caribaeus, G. polynesiensis, G. belizeanus, which are known to cause Ciguatera Fish Poisoning (CFP), were also detected, although their cell densities were low, reaching 2.8 cells/cm² in the dry season and 0.5 cells/cm² in the rainy season.

AUTHOR AFFILIATIONS

1 Institute of Oceanography, Vietnam Academy of Science and Technology, Khanh Hoa, Vietnam
2 Center for High Technology Research and Development, Vietnam Academy of Science and Technology, Hanoi, Vietnam

CITATION

Ho VT, H'Yon NB, Le HP, Phan MT and Phan KH (2025) Study on Potentially Toxic Benthic Dinoflagellate Assemblages on Dead Corals in Nha Trang Bay, Vietnam. Environmental Science Archives 4(2): 713-719.

REFERENCES

Bomber JW, Guillard RRL and Nelson WG (1988) Role of temperature, salinity, and light in seasonality, growth, and toxicity of ciguatera-causing Gambierdiscus toxicus Adachi et Fukuyo (Dinophyceae). Journal of Experimental Marine Biology and Ecology 115: 53–65.

Chateau-Degat M-L, Chinain M, Cerf N et al. (2005) Seawater temperature, Gambierdiscus spp. variability and incidence of ciguatera poisoning in French Polynesia. Harmful Algae 4: 1053–1062.

Chinain M, Darius HT, Ung A. et al., (2010) Ciguatera risk management in French Polynesia: The case study of Raivavae Island (Australes Archipelago). Toxicon 56: 674–690.

Chu VT (2002) Data on species composition and distribution of harmful marine ephiphytic microalgae living on coral reefs in the northern Vietnam. Journal of Biology 24(2): 22–30.

Clausing RJ, Ben Gharbia H, Sdiri K et al. (2024) Tissue distribution and metabolization of Ciguatoxins in an herbivorous fish following experimental dietary exposure to Gambierdiscus polynesiensis. Marine Drugs 22: 14.

Costa PR, Estevez P, Castro D et al. (2018) New Insights into the Occurrence and Toxin Profile of Ciguatoxins in Selvagens Islands (Madeira, Portugal). Toxins 10: 524.

De Azevedo Tibiriçá CED, Sibat M, Felício L et al. (2020) Diversity and toxicity of the genus Coolia Meunier in Brazil, and detection of 44-methyl Gambierone in Coolia tropicalis. Toxins 12: 327.

Estevez P, Castro D, Leão-Martins JM et al. (2021) Toxicity screening of a Gambierdiscus australes strain from the Western Mediterranean Sea and identification of a novel maitotoxin analogue. Marine Drugs 19: 460.

Faust MA (1995) Benthic, toxic dinoflagellates: An overview. Harmful Marine Algal Blooms. Technique at Documentation - Lavoisier, Intercept Ltd., pp.847–854.

Fernández-Herrera LJ, Núñez-Vázquez EJ, Hernández- Sandoval FE et al. (2024) Morphological, toxicological, and biochemical characterization of two species of Gambierdiscus from Bahía de La Paz, Gulf of California. Marine Drugs 22: 422.

Friedman MA, Fernandez M, Backer LC et al. (2017) An updated review of Ciguatera Fish Poisoning: Clinical, Epidemiological, Environmental, and Public Health Management. Marine Drugs 15: 72.

Gómez F, Qiu D, Lopes RM et al. (2015) Fukuyoa paulensis gen. et sp. nov., a new genus for the globular species of the dinoflagellate Gambierdiscus (Dinophyceae). PLoS ONE 10, e0119676.

Ho VT and H’Yon NB (2019) First report of Gambierdiscus caribaeus and G. carpenteri (Dinophyceae) from Nha Trang Bay, South Central Vietnam. Journal of the Marine Biological Association of India 60(2): 5–11.

Ho VT and H’Yon NB (2021) Study of marine sand-dwelling dinoflagellate with four new records from the central south coast of Vietnam. Bangladesh Journal of Plant Taxonomy 28(2): 367–378

Ho VT and Nguyen NL (2008) Composition and density of benthic dinoflagellates from North Danger Reef and Jackson Atoll, Spratly Islands. Proceedings of the Conference on the Results of the Philippines-Vietnam (JOMSRE-SCS I to IV), pp. 65–70. Silliman University Press.

Ho VT and Nguyen NL (2009a) The genus Gambierdiscus Adachi et Fukuyo 1979 (Dinophyta) from Vietnam. Vietnam Journal of Marine Science and Technology 1 (9):199–213.

Ho VT and Nguyen NL (2009b) Benthic dinoflagellates of Con Dao coastal waters (Ba Ria - Vung Tau). Collection of Marine Research Works XVI: 215–224.

Ho VT and Nguyen NL (2014) Morphology and distribution of the three epiphytic dinoflagellate species Coolia monotis, C. tropicalis, and C. canariensis (Ostreopsidaceae, Gonyaulacales, Dinophyceae) from Vietnamese coastal waters. Ocean Science Journal 49(3): 211–221.

Ho VT and Wang P (2023) First report of sand-dwelling dinoflagellate Ceratocorys malayensis (Dinophyceae) from the central south coast of Vietnam. Journal of the Marine Biological Association of India 65(1): 121–124.

Ho VT, Nguyen NL and Doan NH (2010) Benthic dinoflagellates in Vietnamese waters. Publishing House for Science and Technology, Ha Noi.

Holmes MJ (1998) Gambierdiscus yasumotoi, sp. nov. (Dinophyceae), a toxic benthic dinoflagellate from Southeastern Asia. Journal of Phycology 34: 661–668.

Holmes MJ and Lewis RJ (2022) Origin of Ciguateric Fish: Quantitative modelling of the flow of ciguatoxin through a marine food chain. Toxins 14: 534.

Holmes MJ and Lewis RJ (2025) Reviewing evidence for disturbance to coral reefs increasing the risk of Ciguatera. Toxins 17: 195.

Hoppenrath M, Murray SA, Chomérat N et al. (2014) Marine benthic dinoflagellates - Unveiling their worldwide biodiversity. Kleine Senckenberg-Reihe 54, Druckerei Lokay e.K., Reinheim, Germany.

L’Herondelle K, Talagas M, Mignen O et al. (2020) Neurological disturbances of Ciguatera Poisoning: Clinical features and Pathophysiological basis. Cells 9: 2291.

Lehane L and Lewis RJ (2000) Ciguatera: recent advances but the risk remains. International Journal of Food Microbiology 61: 91–125.

Leite IdP, Sdiri K, Taylor A et al. (2021) Experimental evidence of Ciguatoxin accumulation and depuration in carnivorous lionfish. Toxins 13: 564.

Leung PTY, Yan M, Lam VTT et al. (2018) Phylogeny, morphology and toxicity of benthic dinoflagellates of the genus Fukuyoa (Goniodomataceae, Dinophyceae) from a subtropical reef ecosystem in the South China Sea. Harmful Algae 74: 78–97.

Li Z, Park JS, Kang NS et al. (2021) A new potentially toxic dinoflagellate Fukuyoa koreansis sp. nov. (Gonyaulacales, Dinophyceae) from Korean coastal waters: Morphology, phylogeny, and effects of temperature and salinity on growth. Harmful Algae 109: 102107.

Morton SL, Norris DR and Bomber JW (1992) Effect of temperature, salinity and light intensity on the growth and sesonality of toxic dinoflagellates associated with ciguatera. Journal of Experimental Marine Biology and Ecology 157: 79 – 90.

Munday R, Murray S, Rhodes LL et al. (2017) Ciguatoxins and Maitotoxins in extracts of sixteen Gambierdiscus isolates and one Fukuyoa isolate from the South Pacific and their toxicity to mice by Intraperitoneal and Oral Administration. Marine Drugs 15: 208.

Murray JS, Passfield EMF, Rhodes LL et al. (2024) Targeted metabolite fingerprints of thirteen Gambierdiscus, five Coolia and two Fukuyoa species. Marine Drugs 22, 119.

Nguyen-Ngoc L, Doan-Nhu H, Larsen J et al. (2021) Morphological and genetic analyses of Ostreopsis (Dinophyceae, Gonyaulacales, Ostreopsidaceae) species from Vietnamese waters with a re-description of the type species, O. siamensis. Journal of Phycology 57(3): 1059–1083

Nguyen-Ngoc L, Larsen J, Doan-Nhu H et al. (2023) Gambierdiscus (Gonyaulacales, Dinophyceae) diversity in Vietnamese waters with description of G. vietnamensis sp. nov. Journal of Phycology 59(3): 496–517

Pisapia F, Holland WC, Hardison DR et al. (2017) Toxicity screening of 13 Gambierdiscus strains using neuro-2a and erythrocyte lysis bioassays. Harmful Algae 63: 173–183.

Quod JP and Turquet J (1996) Ciguatera in Réunion Island (SW Indian Ocean): epidemiology and clinical patterns. Toxicon 34: 779–785.

Rhodes L, Harwood T, Smith KF et al. (2014) Production of ciguatoxin and maitotoxin by strains of Gambierdiscus australes, G. pacificus and G. polynesiensis (Dinophyceae) isolated from Rarotonga, Cook Islands. Harmful Algae 39:185–190.

Roeder K, Erler K, Kibler S et al. (2010) Characteristic profiles of Ciguatera toxins in different strains of Gambierdiscus spp. Toxicon 56 (5): 731–738.

Soliño L and Costa PR (2020) Global impact of ciguatoxins and ciguatera fish poisoning on fish, fisheries and consumers. Environmental Researcher 182: 109111.

Tester PA, Kibler SR, Holland WC et al. (2014) Sampling harmful benthic dinoflagellates: Comparison of artificial and natural substrate methods. Harmful Algae 39: 8–25.

Wang DZ, Xin YH and Wang MH (2022) Gambierdiscus and its associated toxins: A minireview. Toxins 14: 485.

Xu Y, He X, Lee WH et al. (2021) Ciguatoxin-producing dinoflagellate Gambierdiscus in the Beibu Gulf: First report of toxic Gambierdiscus in Chinese Waters. Toxins 13: 643.

by.png

License: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third-party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Visit for more details http://creativecommons.org/licenses/by/4.0/.

bottom of page